Relationship between high mobility group box-1 protein expression and gut mucosal barrier dysfunction during severe acute pancreatitis / 中国现代普通外科进展

ABSTRACT

Objective:To investigate the relationship between high mobility group box-1 protein (HMGB1) expression and gut mucosal barrier dysfunction during murine severe acute pancreatitis (SAP). Methods:Forty-eight male health adult Wistar rats were divided randomly into Control group and SAP groups. The concentration of plasma D-lactate and the activity of myeloperoxidase (MPO) in the intestinal tissue were determined. The expression of HMGB1 mRNA in intestinal mucosa was detected by reverse transcription polymerase chain reaction (RT-PCR) and the activity of HMGB1 was determined by Western blot. Results:Plasma D-lactate and MPO reached a peak level at 24h (16.41?4.65)?g/mL for Plasma D-lactate and(26.76?3.63)U/g for MPO respectively, (P