Rescue role of Bu-Shen-Yi-Qi Formula for trophoblast apoptosis induced by TNF-?/IFN-? / 中国免疫学杂志
Chinese Journal of Immunology
;
(12)1985.
Artigo
em Chinês
| WPRIM
| ID: wpr-545962
ABSTRACT
Objective:
To investigate the rescue role of Bu-Shen-Yi-Qi Formula on the apoptosis of first trimester trophoblasts induced by TNF-?/IFN-?.Methods:
Cell viability was measured by MTT assay,and cell numbers in sub-G1 phase were determined by FCM.The amount of apoptotic cells was evaluated by TUNEL,caspase-3 enzyme activity was examined by caspase-3 active assay kit.Bcl-2 and XIAP protein expression were detected by Western blot.Results:
(1)Cell viability decreased,but apoptotic cells increased and the cells in sub-G1 phase accumulated when cultured with TNF-? and IFN-? for a 24 h and the reaction became more serious following a 48 h culture.However,cells cocultured with drug-containg serum showed enhanced cell viability,less apoptotic cells and low enzyme activity.Also,cells in sub-G1 phase decreased and Bcl-2,XIAP protein expression were enhanced when the drug-containg serum were added.This change showed time and dosage dependent.Cell viability cultured with blank serum had no significant changes.Conclusion:
Bu-Shen-Yi-Qi Formula rescue trophoblasts apoptosis induced by TNF-? and IFN-?.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Immunology
Ano de publicação:
1985
Tipo de documento:
Artigo
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