Effect of Bisphenol A on Proliferation of Human Uterine Leiomyoma Cells in Culture / 环境与健康杂志

ABSTRACT

Objective To study the effect of environmental estrogen, bisphenol A (BPA), on proliferation of human uterine leiomyoma cells in culture. Methods The primary culture and subculture of the human uterine leiomyoma cells which were identified at each passage by means immunocytochemical staining with a monoclonal anti-?-smooth muscle actin antibody, were performed. The cells (passage 3-5) were incubated with BPA (including three dosimetric systems, 1?10-7 mol/L, 50 ?10-7 mol/L and 100?10-7 mol/L) for 24, 48 and 72 h, and the control cells received only the vehicle (ethanol, 0.1 %, V/V). The proliferation of cultured human uterine leiomyoma cells was analyzed by MTT assay and flow cytometry. Results The limited human uterine leiomyoma cell lines were successfully established. After 24 h, 48 h and 72 h of treatment in 100?10-7 mol/L and 48 h, 72 h, at 50?10-7 mol/L , BPA could enhance the cells proliferation. After 72 h of treatment in 50?10-7 mol/L and 100?10-7 mol/L, BPA promoted cells into S cell cycle phase and elevated mitotic index (P