A simple protocol of DNA sequencing with 10% formamide for dissolving G/C compression
Experimental & Molecular Medicine
;
: 235-237, 1997.
Artigo
em Inglês
| WPRIM
| ID: wpr-54679
ABSTRACT
Formamide has been widely used in urea/polyacrylamide gel to solve the compression problems that are occasionally found during the DNA sequencing of G/C rich regions. In this study, however, 10% formamide was added in annealing solution in stead of adding to the gel. The compressions were unfolded efficiently with a more rapid annealing reaction on ice in the presence of 10% formamide.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
DNA
/
Análise de Sequência de DNA
/
Gelo
Idioma:
Inglês
Revista:
Experimental & Molecular Medicine
Ano de publicação:
1997
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS