Effect of hyperthermia on expression of lung resistance protein and sensitivity to chemotherapeutics in HepG2 cell / 中国现代普通外科进展

ABSTRACT

Objective: To study the changes in the expression of lung resistance protein(LRP) mRNA after multiple hyperthermia and the sensitivity to Adriamycin in HepG2 cell. Methods: HepG2 cell were treated by ten repeated cycles of exposure at 42 degree for 60 minute once a day, and stable HepG2 cell was obtained after hyperthermia. Compared with control HepG2 cell without hyperthermia, Real- time PCR and Western Blot were facilitated to observe the expression of LRP gene, Growth inhibition of Adriamycin to two groups was studied by MTT assay. The sensitivity of cell to Adriamycin was analyzed. Mean fluorescence intensity of ADM were detected by flow cytometry, and the changes of hyperthermia on intracellular concentration of ADM were analyzed. Results: Compared with control HepG2 cell without hyperthermia, the mRNA and protein levels of LRP in HepG2 cell increased 4.01?0.23 and 4.67?0.36 times respectively. This difference was of statistical significance(P