Effects of Fluorouracil Against The Growth and Metastasis of Tumor Cells in Carbon Dioxide Pneumoperitoneum / 中国普外基础与临床杂志

ABSTRACT

Objective To explore the effects of intraperitoneal chemotherapy with fluorouracil(FU) on the growth and metastasis of tumor cells in carbon dioxide(CO2) pneumoperitoneum.Methods Fifty male H-22 mice of clean grade were selected and randomly assigned into 5 groups in each group with 10: simple implantation group,pneumoperitoneum group,pneumoperitoneum and NS group,pneumoperitoneum and low concentration(5.0 g/L) of FU group and pneumoperitoneum and high concentration(10.0 g/L) of FU group.All mice were executed after 11 days to observe the weight and the implantation of tumor in abdominal wall.Then the expressions of proliferating cell nuclear antigen(PCNA) and vascular endothelial growth factor(VEGF) were detected by immunohistochemistry.Results The tumor weight was significantly higher in pneumoperitoneum and high concentration of FU group compared with other groups except pneumoperitoneum and low concentration of FU group(P