PHOSPHOLIPASE A_2 AND ITS RELATIONSHIP WITH ACUTE LUNG INJURY IN ACUTE PANCREATITIS IN DOGS / 第二军医大学学报

ABSTRACT

Acute fulminant pancreatitis was produced in dogs by injection of autobile into the main pancreatic duct.After injection, the phospholipase A2 (PLA2) activities in serum, lung lymph and bronchoalveolar lavage fluid (BAL) were elevated significantly, lung lymph flow and pulmonary transvascular protein clear ance increased progressively, protein content and cell numbers in BAL were significantly higher than those in control animals.Furthermore, lung index, wet to dry lung weight ratio, extravascular lung water to bloodless dry lung weight ratio increased significantly as compared to control animals.Pretreatment with PLA2 inhibitor, chloroquine,blocked the changes mentioned above.This experiment suggests, (1) PLA2 activity in lung lymph fluid as well as in serum and BAL is elevated in acute hemorrhagic pancreatitis.(2) Elevated PLA2 activity may increase the pulmonary vascular permeability.(3) PLA2 is the major factor leading to pulmonary edema in acute hemorrhagic pancreatits.(4) Phagocytes contribute to the lung injury induced by PLA2 to some extent.