Correlation analysis between 5 markers of hepatitis B and HBV-DNA among blood donors with HBsAg negative / 中华疾病控制杂志

ABSTRACT

Objective To discuss the security of negative HBsAg blood by correlation analysis between 5 markers of hepatitis B and HBV-DNA among blood donors with HBsAg negative.Methods 5 markers of hepatitis B were detected by ELISA and HBV-DNA by real-time PCR.Results In 3 398 samples of negative HBsAg blood donors,the total positive rate of HBV-DNA was 2.73%.Among them,the type series of HBsAb(+),HBcAb(+)account 6.59%,the positive rate of HBV-DNA was 5.80%;the type series of HBsAb(+),HBeAb(+),HBcAb(+)account 4.74%,the positive rate of HBV-DNA was 6.83%;the type series of HBcAb(+)alone account 6.24%,the positive rate of HBV-DNA was 5.19%;the type series of HBeAb(+),HBcAb(+)account 5.86%,the positive rate of HBV-DNA was 4.52%;the type series of HBsAb(+)alone account 31.99%,the positive rate of HBV-DNA was 1.56%;the type series of entire negative results account 44.59%,the positive rate of HBV-DNA was 2.11%.Conclusions Blood donors with negative HBsAg still have the risk of infecting HBV,especially when HBcAb is positive alone,or combining with other positive markers such as HBsAb,HBeAb,latent transfusion'infection,the risk was remarkably increased.As a result,we should detect the other markers of hepatitis B simultaneously and carry out real-time PCR technique to screen blood in order to reduce infection rate arising from transfusion.