Ovalbumin immune tolerance factors induce OVA specific peripheral immune tolerance in naive BALB/c mice / 中国免疫学杂志
Chinese Journal of Immunology
;
(12)2001.
Artigo
em Chinês
| WPRIM
| ID: wpr-548095
ABSTRACT
Objective:
To investigate the immunoregulation effect of ovalbumin immune tolerance factors (OVA ITFs).Methods:
Components that were smaller than 3 kD were isolated from the splenic lymphocytes lysates of OVA tolerance mice or naive mice,respectively,named as OVA ITFs or OVA ITFs conttrol.Naive BALB/c mice were divided into 5 groups,group A,B,C,D were injected i.v.by OVA ITFs,OVA ITFs control,splenic lymphocytes from OVA tolerant mice or PBS,respectively,group E as the blank control.The percentages of CD4+CD25+ T cell subpopulation from spleens before and after adoptive transfer were measured with flow cytometer;OVA specific lymphocyte responses were assessed by MTT assay.The levels of IL-10 and TGF-?1 in the culture supernatants were tested by ELISA kits.Results:
For OVA ITFs or splenic lymphocytes from OVA tolerant mice,the percentages of CD4+CD25+ T cell subpopulation from spleens after adoptive transfer were raised significantly compared with that before adoptive transfer (15.32%?1.03% and 15.35%?0.62% vs 9.97%?1.38%,P
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Immunology
Ano de publicação:
2001
Tipo de documento:
Artigo
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