Primary research on the changes of contents and molecular structures of chondroitin sulfate in aortic dissection / 中国现代普通外科进展

ABSTRACT

Objectives:To elucidate the changes on contents and molecular structures of chondroitin sulfate in aneurysmal wall of aortic dissection and disscuss its likely roles in the pathogenesis and progression of aortic dissection.Methods:After glycosaminoglycans in aortae being abstracted,chondroitin sulfate were separated and purified respectively through DEAE Sephacel by ion exchange chromatography and then identified by cellulose acetate electrophoresis.Chondroitin sulfate were hydrolyzed with chondroitinase ABC,and then its disaccharide compositions were analyzed by high performance capillary electrophoresis.Results:Compared with the control group, chondroitin sulfate increased significantly(P