Expression of MAD2 and tumor suppressor gene in the colorectal cancer and clinical significance / 中国癌症杂志

ABSTRACT

Background and purpose:MAD2 is one of the mitotic checkpoints and it is closely associated with tumors.Our aim was to study the expression of gene MAD2 and tumor suppressor gene in colorectal cancer and to demonstrate the relationship between the expression of gene MAD2 and tumor suppressor gene in colorectal cancer,adenoma and normal tissue and to evaluate their clinical significance.Methods:Immunohistochemistry method and real-time fluorescence quantitative PCR were used to analyze the expression of gene MAD2 in colorectal cancer,adenoma and normal tissue.PCR amplifi cation and DNA sequencing were performed to detect the mutations of gene MAD2.p53,p27,p21 and p16 were determined in colorectal cancer and normal tissue by immunohistochemistry methods.Results:The expression of MAD2 in colorectal cancer was obviously higher than in adenoma and normal tissue(66.7% vs 39.6% vs 22.9%) ,there were signifi cant differences among colorectal cancers,adenoma and normal tissue(P