Establishing and evaluating of mouse closed femur fracture / 中国矫形外科杂志

ABSTRACT

[Objective]To develop a standard closed experimental fracture model in mouse femur.[Method]A 0.45 mm stainless-steel rod was introduced into the medullary cavity and the pre-nailed femur shaft was fractured by an impact device,which resulted in a reproducible transverse fracture pattern.The fractures were examined using X-ray Micro-CT,and histological method.[Result]Transverse fractures were created in 91.67% of the experimental mice,and the fracture healing was typical secondary fracture healing.[Conclusion]The standard closed fracture model in this experiment,whose healing was typically secondary fracture healing,can be obtained easily and used in study of fracture.