SIMULTANEOUS DETERMINATION OF CHROMIUM, MANGANESE AND COPPER IN SERUM USING FLAMELESS ATOMIC ABSORPTION SPECTROPHOTOMETER / 营养学报

ABSTRACT

A sensitive method for the determination of Cr, Mn and Cu in serum sample by flameless atomic absorption spectrophotometry is described. It requires only 0.1-0.2 ml serum sample. The method involves low temperature ashing of serum, 2-10 fold dilution with 0.01N HNO3 and injection of 20?l aliquot into graphite furnace. The known amounts of trace metals arc added to different diluted serum samples, and the recovery rates are 90-109%. The coefficients of variance for within-run and day-to-day values are 1.5-2.8% and 2.0-10.0% respectively. The serum Cr, Mn and Cu levels were determined in 79 normal subjects. 80% of the values are distributed within 0.8-1.45 ?g/ml(Cu), 0.04-0.08 ?g/ml(Mn), 0.009-0.022 ?g/ml(Cr). It is suggested that the method described is available for a mass survey in a short time and is valualole to detect the trace metal concentation in serum in clinical laboratory.