Study on Serum Dilution in Detecting Anti HBc-IgM with ELISA / 第三军医大学学报
Journal of Third Military Medical University
; (24)1983.
Article
em Zh
| WPRIM
| ID: wpr-549194
Biblioteca responsável:
WPRO
ABSTRACT
A study on the optimal dilution of serum to detect anti HBc-IgM with ELISA. was carried out. Samples were diluted to 10-1to 10-7. Among 26 samples of normal individuals, 22 were negative, but two samples of 10-1 dilution, one sample of 10-2, and one sample of 10-1-10-3 were positive though the control wells were all negative. 42 samples of the patients with variours types of hepatitis B were examined. It was found that when serum dilution was 10-1, only 6 cases were positive; when the dilution was 10-2, 20 cases were positive; when the dilutions were 10-3, 5?10-3, and 10-4, high positive rates were obtained and they were 88.1%, 78.5%, and 69% respectively; and when the samples were further diluted, the positive rate decreased.In order to get goodfresults and minimize false results, either false negative or false positive, it is suggested that 5?10-3 be the optimal dilution.
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Índice:
WPRIM
Idioma:
Zh
Revista:
Journal of Third Military Medical University
Ano de publicação:
1983
Tipo de documento:
Article