THE CONSTRUCTION OF HYBRID pBR322: RSF1010 / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12)1981.
Artigo
em Chinês
| WPRIM
| ID: wpr-549202
ABSTRACT
The plasmids pBR322 and RSF1010, extracted from E.coli HB802 and E. coli C600 respectively were treated with restrictive endonuclease EcoRI and ligated with T4 DNA ligase. C600 strains were then transformed with the hybrid plasmid, and thus 253 transformants were obtained.The figures of gel elctrophoresis and electromicrograph proved that PSMM1 was a pBR322 RSF1010 composite hybrid.In E. coli C600 PSMM1 obtained resistance against AP, Tc and Sm.The orientation of pBR322 and RSF1010 in hybrid PSMM1 and the failure of introduction of this hybrid into P.Putida AC10 were discussed
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Academic Journal of Second Military Medical University
Ano de publicação:
1981
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS