Ultramicro-quantitative Determination of Tissue NAD~+ / 第三军医大学学报

ABSTRACT

This article is to report an ultratnicro-quantitative method to determine the tissue content of NAD+. The cycling mixture of nitrotetrazolium blue chloride, phenagene methosulfate, lactate and lactic dehydrogenase is used. The procedure of this assay is very simple and easy to perform. It is sensitive enough to detect as little an amount as 1?10-11 mole per ml of NAD+.