Method for Determination of Plasma Catecholamine / 第三军医大学学报

ABSTRACT

This paper is to introduce a new method for the determination of the plasma level of catecholamine. The method consists of two steps. Firstly, catecholamine is seperated and purified with aluminium oxide and sephadex G-10. Secondly, the purified catecholamine is oxidized into trihydrooxyindole and the amount is determined by fluorimetry.This method is rather simple and can be handled easily. It can be used either in research or in clinical work. The procedures of the method and, the relevant factors to the accuracy of the method were discussed in detail and the data obtained from experimental animals, normal persons and different patients were presented.