The Quality Control of Immunofluorescent Antibody Test and Anaerobic Culture in Study of Bacteroides and Fusobacterium / 第三军医大学学报

ABSTRACT

A comparative study between the indirect immunofluorescent antibody test and anaerobic culture in the study of Bacteroides fragilis, Bacteroides melani-nogenicus, and Fusobacterium nucleatum in 374 clinical specimens was carried out. The data concerning the quality control of the two tests including the rate of correlation, the difference of the sensitivity and specificity,the predictive values, and the time of antiserum preservation were analyzed. It was found that immunofluorescent antibody test had a higher rate of detection than anaerobic culture. It can be cartain that if immunofluorescent antibody test is negtive, it is more likely that the anaerobic bacteria are abscent. The rate of correlation between the two tests was 88.5~95.99%. In addition, immunofluorescent antibody test can be used to verify the quality of anaerobic culture. It was found that our routine culture for B, fragilis was inperfect and waited for improvement.