Isolation of C1q in Human Serum with Ami-human C1q McAb Affinity Chromatography / 第三军医大学学报

ABSTRACT

This paper is to report a procedure to isolate C1q in human serum with anti-human Clq McAb affinity chromatography.This procedure is simple and rapid,which can be performed within 24 to 48 hr The yield of Clq was 7~10 mg per 100ml serum.The preparations were immunoglobulin free as judged by double diffusion and immunoelectrophoresis tassay and exhibited typical C1q subunit structure in SDS-PAGE.After biological activity evaluation with red cell agglutination and latex agglutination assay,this C1q preparation was found to be satisfactory in its activity.The anti-human Clq McAb sepharose 4B column can be used repeatedly and stored in 4℃ for approximately one year without loss of absorption activity.