Isolation and Identification of cytopathogenic Effect Strains of Epidemic Hemorrhagic Virus in Vero-E_6 Cell Culture / 第三军医大学学报
Journal of Third Military Medical University
;
(24)1984.
Artigo
em Chinês
| WPRIM
| ID: wpr-549605
ABSTRACT
Five strains out of the 8 of epidermic hemorrhagic fever(EHF)virus isolated from the serum of the acute phase of EHF patients in Nanchong, Sichuan were found to have cytopathogenic effect(CPE)through 4th to 10th passages in Vero-E6 cell culture. The biological, physico-chemical and antigenic characteristics of HFN-04 and HFN-19, 2 out of the 5 CPE strains, were identified. The results were as follows;( 1 ) The CPE development and the dynamic proliferation of the virus areconsistent. ( 2 ) The main biological and physico-chemical characteristics of the 2 CPEstrains arc the same as those of EHF virus.( 3 ) When paired samples of EHF patients' serum were tested with the slide antigens of E6 cells infected with these CPE strains, all the convalescent serum demonstrated an increase by 4 fold or more of the IFA. titer.( 4 ) The CPE of the virus can be neutralized by EHF patient's serum or EHF and KHF antisera, but not by normal rabbit serum or the multivalent serum of Reovirus type 1-3.( 5 ) The E6 cells infected by CPE strains of virus can almost fully absorbthe specific antibodies in EHF patients or in EHF and KHF antisera.( 6 ) The suspension of the mouse brain infected by CPE strains can formpositive agglutination reaction with the sentitized blood cells by EHFvirus strain A9 McAb 25-1.On the basis of our observation, it can be concluded that at least certain strains of EHF virus can produce CPE in Vero-E6 cell culture.
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DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Idioma:
Chinês
Revista:
Journal of Third Military Medical University
Ano de publicação:
1984
Tipo de documento:
Artigo
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