EFFECTS OF TOTAL GLUCOSIDES OF PAEONY ON CHEMILUMINESCENCE OF PERITONEAL MACROPHAGES IN RATS / 中国药理学通报

ABSTRACT

Influences of the concentrations of luminol, zymosan & cells and opsonizing time of zymosan on luminol-dependent chemiluminescence( LDCL ) of peritoneal macrophages ( PM?) in rats were investigated by the method of orthogonal analysis. The result showed that the best levels were 100?mol/L of luminol, 10 mg/ml of zymosan, 5? 10~6/ml of cells and 45 min for opsonizing time. The LDCL of PM? was enhanced by incubation with TGP for 12 h in a concentration-dependent manner in the range of 0.09-2.25 ?g/ml of TGP, but LDCL was diminished with the concentration of 11.25 ?g/ml of TGP . The concentration-effect curve seems to be bell-shaped. The data suggested that TGP had two-sided regulatory effects on the LDCL of PM? in rats, depending on the concentration used.