Gene Expression Regulation by Agonist-Independent Constitutive Signaling of Melanocortin-1 Receptor
Endocrinology and Metabolism
;
: 179-184, 2014.
Artigo
em Inglês
| WPRIM
| ID: wpr-55020
ABSTRACT
BACKGROUND:
Melanocortin-1 receptor (Mc1r), a key signaling receptor for melanogenesis, has been reported to mediate migration of B16F10 melanoma cells. Interestingly, this activity appears to be a part of the constitutive signaling of Mc1r.METHODS:
We carried out small interfering RNA-mediated knock-down of Mc1r on murine melanoma B16F10 cells and performed microarray analysis to characterize changes in the gene expression profile.RESULTS:
We isolated 22 and four genes whose expression decreased and increased, respectively, by 2.5-fold or higher as the result of Mc1r knock-down. Several down-regulated genes have been proposed to be involved in cell migration. Among these genes are several members of the chemokine gene family.CONCLUSION:
We provide a gene set for further functional analyses of Mc1r. The Mc1r target genes we present may be particularly relevant for understanding the ligand-independent activity of Mc1r. Further examination of the mode of action may lead to novel strategies in regulating the migration and metastasis of melanoma cells.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Movimento Celular
/
Regulação da Expressão Gênica
/
Genes vif
/
Quimiocinas
/
Receptor Tipo 1 de Melanocortina
/
Análise em Microsséries
/
Transcriptoma
/
Melanoma
/
Metástase Neoplásica
Limite:
Humanos
Idioma:
Inglês
Revista:
Endocrinology and Metabolism
Ano de publicação:
2014
Tipo de documento:
Artigo
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