Experimental study Detecting Entroviruses RNA With the Method of cDNA: RNA Spot Hybridization / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12)1985.
Artigo
em Chinês
| WPRIM
| ID: wpr-550631
ABSTRACT
Taking Coxsackie B3 virus cDNA labelled by Biotin as detecting probes and 25 kinds of standard enteroviruses as detected viusr, a spot nucleic acid hybridization method has been established to detect enterovirus RNA gene. The method has simple operation and its sensitivity is 50-80 TCID50 spot. Reagent is safe and stable, and may be stored for long period. pCBHⅢ/35.51 probe used has wider hibridization spectrum to enteroviruses; pCBⅢ/29 only reacts with RNA of Coxsackie B3 virus in strict condition. Consequently, the hybridization character which these probes possess is suitable to determining enlerovirus in clinical samples.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Academic Journal of Second Military Medical University
Ano de publicação:
1985
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS