Corneal neovascularization induced by controlled-release of interleukin-2 in rabbits / 眼科

ABSTRACT

Corneal neovascularization(CNV)is one of the main causes of corneal allograft rejection.We have developed a CNV model in rabbits by controlled- release of IL-2.Its characterization and developmental processes were observed and its histological mechanism was explored.CNV was revealed through staining of erythrocytes within capillaries with 3,3′-diaminoben- zidine(DAB).Ethylene vinyl acetate copolymer (EVA-40)pellets incorporated with IL-2 100u,200u, 500u and control blank pellets were implanted in corneal mid-stromal pocket 2mm from the upper limbus.Biomicroscopic evaluation revealed that IL-2 induced CNV in a dose-dependent manner.IL-2 500u induced CNV in all the experimental eyes with good consistency and reproducibility.Histopathologi- cally,the IL-2 induced CNV was closely associated with mononuclear cell infiltration.This model fairly closely mimicked the immunologically mediated CNV. The architecture and perfusion of CNV was clearly revealed by the red blood cells stained with DAB.