CONSTRUCTION OF SALMONELLA STN-DEFICENT MUTANTS AND DETECTION OF THEIR VIRULENCE / 解放军医学杂志
Medical Journal of Chinese People's Liberation Army
;
(12)1982.
Artigo
em Chinês
| WPRIM
| ID: wpr-551863
ABSTRACT
The plasmids of recombinant suicide vector containing the truncated stn gene and the deleted stn gene were constructed. The Salmonella stn-deficient mutant was prepared by homologous recombination between the mutant stn gene in the recombinant suicide vector and the wild-type gene in the chromosome of S. Typhimurium 2000. The detection of biological activity of stn gene production in those mutants indicated that Salmonella stn-deficient mutants evoked significantly less fluid secretion in mouse intestinal loops compared to that seen with wild-type Salmonella. Upon oral challenge of mice, the fifty percent lethal dose of the Salmonella stn-deficient mutants was greater than that for the wild-type bacteria. Those studies showed that the stn gene is very important factor in the pathogenesis of salmonellosis.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo diagnóstico
Idioma:
Chinês
Revista:
Medical Journal of Chinese People's Liberation Army
Ano de publicação:
1982
Tipo de documento:
Artigo
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