Effect of quercetin on cell cycle and nitroxide in hydrogenperoxide- induced of endothelial cells / 中国药理学通报

ABSTRACT

AIM To study the effects of quercetin (Que) on cell cycle and nitric oxide in H2O2-induced the human umbilical vein endothelial cell line(ECV- 304). METHODS The experiment were performed in culture of H2O2-induced human umbilical vein endothelial cell line(ECV-304) in vitro. Cell viability was assessed by MTT assay and cell cycle was observed by flow cytometry. Nitroxide(NO) of ECV304 was monitored as No2- with colorimetry. RESULTS H2O2 inhabited the ECV-304 prolifteration. Preincubation of ECV-304 with one for 24 h before H2O2 exposure significantly increased the Cell viability and S-phase and G2M-phase cells. one reduced lactate dehydrogenase(LDH) and increased the level of nitric oxide in H2O2-induced ECV-304. CONCLUSION These results demonstrate that one can produce the protective action on H2O2-induced cultured ECV-304 and its effect of action may be related to level of nitric oxide.