Effects of X-ray irradiation on focal adhesion kinase in vascular smooth muscle cells / 中华放射肿瘤学杂志

ABSTRACT

Objective To investigate the signaling transduction mechanisms of inhibiting effect of radiation on vascular smooth muscle cells(VSMC).Methods Cell culture in vitro and semi quantitative reverse transcription polymerase chain reaction(RT PCR) were used to observe the dynamic change of VSMC focal adhesion kinase(FAK) on the mRNA expression level and result of the control group as compared to those of the irradiation ones at 2.5,10,15,20 Gy.Results Dose of 2～20?Gy irradiation down regulated the expression of FAK mRNA. At hour 48 after 2～20?Gy irradiation,the FAK mRNA expressions were all less than the control ones(F=364.21,P