THE INFLUENCE OF DIFFERENT PROCESSING TECHNIQNE FOR ALLOGENEIC BONE ON PROLIFERATION AND ACTIVITY OF OSTEOBLASTS / 解放军医学杂志

ABSTRACT

To study the influence of different processing technigue in preparing allogeneic bone on proliferation rate and activity of rabbit osteoblasts in vitro, Osteoblasts were cultured combined with allogeneic bone prepared with different processing techniqne. Cell proliferation and activity were assessed 48 and 96h with MTT colorimetry and assay of alkaline phosphatase activity. The results showed that freezed bone (FB) and lyophilized bone (LB) inhibited cell growth and activity, while mineralized bone matrix (DBM),bone matrix gelatin (BMG), and autolyzed antigen extracted allogeneic bone(AAA) enhanced them. With the exception of FB and LB, the three others were biocompatible for osteoblasts.