Effect of eupolyphaga seu steleophaga on proliferation of autosomal dominant polycystic kidney disease cyst-lining epithelial cells / 第二军医大学学报

ABSTRACT

Objective:To observe the effect of eupolyphaga seu steleophaga(ESS) on the proliferation of autosom al dom inant polycystic kidney disease(ADPKD) cyst- lining epithelial cells.Methods:ESS serum and control serum were obtained from SD rats gastrogavaged with ESS water solution and physiological solution.ADPKD cyst- lining epithelial cells were treated with RPMI16 4 0 containing(1) EGF0 ,2 .5 ,5 ,10 ,2 0 ng/ ml;(2 ) ESS serum and control serum(1% ,2 .5 % , 5 % ) ;(3) EGF10 ng/ ml,ESS serum and control serum(1% ,2 .5 % ,5 % ) .The cell proliferation was determined by Brdu incorporation assay. Results:EGF had a stimulating effect on the ADPKD cyst- lining epithelial cells;the ESS serum significantly inhibited the proliferation of ADPKD cyst- lining epithelial cells compared with control serum;ESS serum also significantly inhibited the proliferation of ADPKD cyst- lining epithelial cells activated by EGF(10 ng/ m l) com pared with control serum.All the effects were dose- dependent.Conclusion:Eupolyphaga seu steleophaga may prolong the progression of ADPKD via the inhibitory effect on the proliferation of cyst- lining epithelial cells. [