CONSTRUCTION OF THE MUTANTS OF HUMAN PEPTIDE ANTIBIOTICS hPAB-? AND ITS RECOMBINANT BACULOVIRUS / 解放军医学杂志
Medical Journal of Chinese People's Liberation Army
;
(12)1983.
Artigo
em Chinês
| WPRIM
| ID: wpr-552838
ABSTRACT
The aim was to determine the antimicrobial activities of peptide antibiotics hPAB ? and construct the recombinant baculovirus of its mutants. Four mutants of hPAB ? were designed based on molecular autosyndetic modeling and its genes were cloned by PCR. The transfer plasmid and recombinant baculovirus were constructed. The results showed that the hPAB ? cloned previously, is a good peptide antibiotics. Four mutant genes of hPAB ? were inserted into pFAST HTa plasmid and the recombinant pFAST hPAB ? were screened by restriction enzymes analysis and DNA sequencing. The recombinant baculovirus was obtained after transforming pFAST hPAB ? into Escherichia coli DH10Bac. Our work lays a good foundation for further research.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Avaliação de Tecnologias em Saúde
Idioma:
Chinês
Revista:
Medical Journal of Chinese People's Liberation Army
Ano de publicação:
1983
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS