Improvement on micro-manipulation of fertilized eggs of mice / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12)2001.
Artigo
em Chinês
| WPRIM
| ID: wpr-553158
ABSTRACT
Objective:
To improve the micro-manipulation of mouse fertilized eggs,promoting its survival rate and productivity in pseudo-pregnant mice.Methods:
DNA fragments of definite concentration and size were used for micro-manipulation with conventional and improved methods, then their survival rate of manupulated eggs and productivity of pseudo-pregnant mice were analyzed and compared.Results:
With improved methods, the survival rate of eggs increased from 60.3% to 79.5%, the productivity of pseudo-pregnant mice and offspring increased from 35.7% to 56.8% and 10.1% to 14.1% respectively.Conclusion:
The improved methods of micro-manipulation can be used in transgenic mouse production.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Academic Journal of Second Military Medical University
Ano de publicação:
2001
Tipo de documento:
Artigo
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