MOLECULAR MECHANISM OF PATHOGENESIS OF HEPATITIS C VIRUS / 解放军医学杂志

ABSTRACT

The pathogenesis of hepatitis C virus (HCV) is different from that of DNA virus and retrovirus. The epitope inducing neutralizing antibody in high variable region 1 (HVR1) is the reason for escape of host immune surveillance, evolution of chronic infection, and also difficulty of producing wide spectrum vaccine. Mimotope selected by using phage display technique is an alternative to solve this problem. The study on the binding protein to non coding region of HCV RNA is helpful to uncover the regulation of replication and translation of HCV genome. A study of the specific receptor of HCV is important to elucidate the mechanism of how HCV enter the hepatocyte. Yeast two hybrid is a useful technique to study the protein protein interactions between HCV and hepatocyte. Suppressive subtraction hybridization is an efficient method for identification of genes of interest transactivated by HCV structural and non structural proteins. All these studies will finally resulted in the full understanding of HCV pathogenesis, and will supply unique opportunity for the development of a new therapy for HCV infection.