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Separation,culture and identification of neural stem cells from rat embryo / 第二军医大学学报
Academic Journal of Second Military Medical University ; (12)1985.
Artigo em Chinês | WPRIM | ID: wpr-553879
ABSTRACT
This study is to set up a method for separating,culturing and identifying neural stem cells from cortex in rat embryo,observing their characteristics of multipotentiality and differentiation in vitro. By using serum-free cell culturing and clone technology,the stem cells were isolated,cultured and passaged from cortex in 14-day old rat embryo. The expression of Nestin antigen of brain cells were investigated by immunocytochemistry and H-E staining. It proved that cells separated from cortex in rat embryo possessed the potential to form clones and express nestin special antigen,the differentiated cells could express special antigen of neurons,astrocytes,and oligodendrocytes,they were stem cells of central nervous system.

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Tipo de estudo: Estudo diagnóstico / Estudo prognóstico Idioma: Chinês Revista: Academic Journal of Second Military Medical University Ano de publicação: 1985 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Tipo de estudo: Estudo diagnóstico / Estudo prognóstico Idioma: Chinês Revista: Academic Journal of Second Military Medical University Ano de publicação: 1985 Tipo de documento: Artigo