An experimental research on inhibition of immediate and delayed type hypersensitivity by the 70% ethanolic extract from euonymus alatus / 中国药理学通报

ABSTRACT

ABSTRACT AIM To explore the restrained activities of the 70% ethanolic extract from euony-mus alatus(EAL) on the immediate and the early and late phases of delayed type hypersensitivity (DTH), and explore its mechanisms of action. METHODS EAL was pre-incubated with rat peritoneal mast cells and isolated cavia porcellus ile-um, respectively. Its effects on histamine release were elicited by compound 48/80 and ileum constriction by 5-PC. Obserseved the effects of EAL, which separated total flavanid from, it HT were observed. In mouse DTH models upon challenge with SRBC or was on the early and late phases by administration after antigen challenge. RESULTS EAL had a dependent restraint-effect to release the histamine from rat peritoneal mast cells and showed a restrained tendency on the cavia porcellus ileum constriction. Meantime, EAL and its total flavanid can restrain the two phases of DTH too. Which were stronger on the late phase than early. CONCLUSION The steroid and flavanid of EAL can restrain the early and late phase of DTH by stabling mast cell membrane and restraing histamine and 5-HT release. Its flavanid is the active component to restrain DTH.