EFFECTS OF EXOGENOUS hTERT TRANSFECTION ON TELOMERIC RESTRICTION FRAGMENT,TELOMERASE ACTIVITY AND ITS SUBUNITS EXPRESSION OF HUMAN EMBROYNIC FIBROBLASTS / 解放军医学杂志

ABSTRACT

In order to explore the effects of exogenous human telomerase reverse transcriptase (hTERT/hTRT/hEST2) on telomeric restriction fragment (TRF), telomerase activity and its subunits expression in human embryonic fibroblasts (hEFs), hTERT sense eukaryotic expression vector pIRES2 EGFP hTERT was constructed with DNA recombinant technique and then transfected into primary hEFs by Lipofectin method. TRF length, telomerase activity and changes in telomerase subunits expression were examined and evaluated in transfected and untransfected cells. The results showed that telomerase activity in pIRES2 EGFP hTERT transfected cells (hEF EGFP) was significantly higher than that in untransfected hEFs and vacant vector transfected cells (hEF EGFP) ( P