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SCRAPING METHOD FOR ISOLATION OF RAT PARIETAL CELLS / 解放军医学杂志
Medical Journal of Chinese People's Liberation Army ; (12)2001.
Artigo em Chinês | WPRIM | ID: wpr-554222
ABSTRACT
Isolation of rat gastric mucosal cells was performed with scraping method followed by protease and collagenase digestion. Parietal cells were observed and identified with phase-contrast microscopy, light microscopy with hematoxylin and eosin staining, electron microscopy, fluorescence microscopy, and also immunocytochemistry technique. The diameters of parietal cells were measured. The results showed that the mucosal cells were successfully isolated, and it was confirmed that the parietal cells were the biggest cells among all the mucosal cells.

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Medical Journal of Chinese People's Liberation Army Ano de publicação: 2001 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Medical Journal of Chinese People's Liberation Army Ano de publicação: 2001 Tipo de documento: Artigo