THE ROLE OF ARGININE IN IMMUNE-ENHANCING EFFECT VIA LIVER AND ITS MECHANISM / 营养学报
Acta Nutrimenta Sinica
;
(6)1956.
Artigo
em Chinês
| WPRIM
| ID: wpr-554265
ABSTRACT
Objective:
To study if the immune-enhancing effect of Arg was mediated via liver.Methods:
The direct effect of Arg on T cell proliferation was measured by 3H-TdR incorporation. Rat hepatocytes were primarily cultured in serum-free DMEM/F12 medium, and then cultured in medium containing Arg(?mol/L0,7.5,75,750,7 500), and the supernatant was collected at 0, 24, 48, 72 hours, and added to splenocytes culture, and T cell proliferation, NK cell activity and IL-2 activity,〔Ca 2+〕i were measured respectively.Results:
Arg had no direct effect on splenocyte proliferation. The hepatocyte culture supernatant significantly increased the lymphocyte〔Ca 2+〕i ,IL-2 activity and T lymphocyte proliferation; 7 500 ?mol/L was most effective.Conclusion:
Arg may enhance immune function via secretion of bioactive molecules by hepatocytes.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Acta Nutrimenta Sinica
Ano de publicação:
1956
Tipo de documento:
Artigo
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