Characteristic response of CD4~+ cell in chronic Schistosoma japonicum in fection by gene expression profiling / 中华传染病杂志

ABSTRACT

Objective The changes of cytokine expression on a genomic scale in CD4 + cell of mice c hronically infected with Schistosoma japonicum and their roles in the pathog enesis of schistosomiasis were studied. Methods CD4 + cells were isolated from sp le ens of mice 13 wk infected with Schistosoma japonicum. We used the cDNA micr oarr ay technique to explore the cytokine gene expression profile of CD4 + cells f ro m normal and chronic infected mice. Three-color flow cytometry was performed to study intracellular protein levels of IFN-?、IL-4 of CD4 + cell. Results In the CD4 + cell of chronically infected mice, IL-4 was remarkably increase d at both transcription and protein expression levels (P