Evaluation of Nested Multiplex PCR in the Diagnosis of Malaria Infection / 대한진단검사의학회지
The Korean Journal of Laboratory Medicine
;
: 447-451, 2002.
Artigo
em Coreano
| WPRIM
| ID: wpr-55449
ABSTRACT
BACKGROUND:
A peripheral blood smear has been the gold standard method for the diagnosis of malaria infection. Recently, many other methods have been introduced, although having inferior sensitivity and specificity to peripheral blood smears. We evaluated Neodin malaria PCR kit and its applicability in clinical settings.METHODS:
Samples from seventy patients who visited Korea University hospital were used for evaluation. DNA from EDTA blood was tested in nested multiplex PCR and 470 bp for Plasmodium vivax or 340 bp for Plasmodium falciparum was confirmed after electrophoresis. The detection limit was determined by dilution of malaria positive blood with normal blood.RESULTS:
Thirty-five cases of P. vivax and 10 cases of P. falciparum were noted. Except for a case of falciparum malaria, all positive cases were consistent with the peripheral blood smear results. Detection limit was 3.6 parasite/microL.CONCLUSIONS:
Neodin malaria nested multiplex PCR has high sensitivity and the ability for species discrimination and may be available in the diagnosis of malaria infection.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plasmodium falciparum
/
Plasmodium vivax
/
DNA
/
Reação em Cadeia da Polimerase
/
Sensibilidade e Especificidade
/
Ácido Edético
/
Diagnóstico
/
Discriminação Psicológica
/
Eletroforese
/
Limite de Detecção
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
País/Região como assunto:
Ásia
Idioma:
Coreano
Revista:
The Korean Journal of Laboratory Medicine
Ano de publicação:
2002
Tipo de documento:
Artigo
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