Recent development of phage display technique and its application in the study of pathogenesis of viral hepatitis / 解放军医学杂志

ABSTRACT

Phage display is a technique that combines the expressed protein/peptide on the surface of a bacteriophage and its affinity binding capacity. The pⅢ and pⅧ genes are both suitable target sites for insertion of foreign cDNA fragment. In the early stage of this technique, it was mainly used for screening and identification of single chain antibody(scFv) and/or random peptide. Along with the construction and application of cDNA library for phage display, the application of this technique has been significantly broadened. At present phage display is one of the most important and efficient techniques for the study of protein-protein interaction, and DNA/RNA-binding proteins. Even non-protein small molecule-binding proteins could be defined by this technique. Recent progress has demonstrated that the phage display technique is a powerful tool for the investigation of autoantigen, tumor marker, proteins related to signal transduction, and even regulatory proteins involved in the gene expression of hepatitis viruses.