Apoptin induces G_2-M arrest in cancer cells / 北京大学学报(医学版)
Journal of Peking University(Health Sciences)
;
(6)2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-555083
ABSTRACT
Objective:
To further study the effect of apoptin in inducing cancer cell specific apoptosis and the possible applications in cancer therapy.Methods:
Apoptin gene was amplified by PCR and inserted into pcDNA3.1(+) with a FLAG tag in front of the multi-cloning-site. Apoptin gene with the FLAG tag was sub-cloned into an adenovirus vector. Several cancer cell lines were transfected with pcDNA3. 1/FLAG/apoptin or infected with apoptin containing recombinant adenoviruses to study the morphologic changes. Ad/apoptin infected cells were also analyzed by flowcytometry after staining with PI.Results:
Expressed apoptin was localized in the nucleus of cancer cells. Chromatin condensation occurred 2 or 3 days after Ad/apoptin(+) infection. Cell number in G 2-M phase increased dramatically after Ad/apoptin(+) infection.Conclusion:
Apoptin can induce cell cycle G 2-M arrest and chromatin condensation in cancer cells.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Journal of Peking University(Health Sciences)
Ano de publicação:
2004
Tipo de documento:
Artigo
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