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Apoptin induces G_2-M arrest in cancer cells / 北京大学学报(医学版)
Journal of Peking University(Health Sciences) ; (6)2004.
Artigo em Chinês | WPRIM | ID: wpr-555083
ABSTRACT

Objective:

To further study the effect of apoptin in inducing cancer cell specific apoptosis and the possible applications in cancer therapy.

Methods:

Apoptin gene was amplified by PCR and inserted into pcDNA3.1(+) with a FLAG tag in front of the multi-cloning-site. Apoptin gene with the FLAG tag was sub-cloned into an adenovirus vector. Several cancer cell lines were transfected with pcDNA3. 1/FLAG/apoptin or infected with apoptin containing recombinant adenoviruses to study the morphologic changes. Ad/apoptin infected cells were also analyzed by flowcytometry after staining with PI.

Results:

Expressed apoptin was localized in the nucleus of cancer cells. Chromatin condensation occurred 2 or 3 days after Ad/apoptin(+) infection. Cell number in G 2-M phase increased dramatically after Ad/apoptin(+) infection.

Conclusion:

Apoptin can induce cell cycle G 2-M arrest and chromatin condensation in cancer cells.

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Journal of Peking University(Health Sciences) Ano de publicação: 2004 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Journal of Peking University(Health Sciences) Ano de publicação: 2004 Tipo de documento: Artigo