Cultivation and identification of porcine skin fibroblasts / 第三军医大学学报

ABSTRACT

Objectives To establish an ideal cellular model for the study of infection of porcine endogenous retrovirus in intro and in vivo and to lay the foundation for evaluating the biological safety of xenotransplantation. Methods Porcine skin fibroblasts were cultured by tissue pieces, cold trypsin, and heat trypsin, respectively. The merit and defect of these methods were analyzed. Results Porcine skin fibroblast line was established and the tissue piece method was superior to trypsin methods. Conclusion Culture of porcine skin fibroblasts with tissue pieces is superior to trypsin method, with the characteristics of low cost and easy operation. The established porcine skin fibroblast cell line supplies an ideal cell model for the study of infection of porcine endogenous retrovirus in vitro and in vivo, provides a new method for the evaluation of biological safety of xenotransplantation, and plays an important role in the skin culture model of production of cloned animals.