Promoting effects of interleukin 13on proliferation of murine airway smooth muscle cells / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12)2000.
Artigo
em Chinês
| WPRIM
| ID: wpr-555275
ABSTRACT
Objective:
To investigate the promoting effects of interleukin-13(IL-13)on the proliferation of airway smooth muscle cells(ASMC)in mice and its mechanism.Methods:
Murine ASMC were isolated and subcultured.IL-13,IL-13plus AG1478,IL-13plus neutralizing antibody to TGF-?,IL-13plus neutralizing antibody to EGF,IL-13plus neutralizing antibody to HB-EGF,IL-13plus dexamethasone were added to the media,respectively.MTT and 3 H-TdR incorporation assays were used.Apical media were analyzed for the presence of soluble TGF-?using ELISA.Results:
The D 595 value of MTT assay and the cpm value of 3 H-TdR incorporation assay of IL-13group were significantly higher than those of control group(P
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Academic Journal of Second Military Medical University
Ano de publicação:
2000
Tipo de documento:
Artigo
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