Inhibitory effect of clindamycin on biofilm of mucoid P.aeruginosa in vitro / 解放军医学杂志

ABSTRACT

Objective To investigate the influence of clindamycin (CM) on the biofilm of mucoid P. aeruginosa (PA). Methods A biofilm model of mucoid PA were reproduced in vitro by plate culture method, and it was identified under SEM (scanning electronic microscopy). The viable bacteria were counted by MTT method. The morphology of biofilms was observed under SEM. Results Stable PA biofilms formed after PA was incubated for 7 days. The viable bacteria and biofilm morphology showed little change in single CM or levofloxacin (LFX) drug groups. When CM was combined with LFX, the number of viable bacteria was significantly decreased, and the biofilm structure was damaged, and the remaining bacteria were separated. Conclusion Clindamycin can suppress the biofilm formation of mucoid PA when combined with LFX.