Transplantation of autologous peripheral blood stem cells for the treatment of arteriosclerosis obliterans of lower limb in 26 cases / 解放军医学杂志

ABSTRACT

Objective To observe the clinical efficacy and interrelated factors of autologous peripheral blood stem cells (PBSC) transplantation in the treatment of arteriosclerosis obliterans (ASO) of the lower extremity. Methodes A total of 26 patients with ASO of the lower limb received rhG-CSF 450-600?g/d by hypodermic injection for 5 days to mobilize stem cells. On the sixth day, PBSC were collected by COBE 6.1 Spectra Version with a total amount of 82-118ml, with the number of mononuclear cells (MNC) (718.2-224.6)?10 9 /L. The MNC were injected intramuscularly into the ischemic limbs with intervals of 3cm apart. The clinical and laboratory findings were monitored. Results In 23 out of 26, pain was relieved after 3-21 days (88.5%), in 24 patients coldness was ameliorated after 2-28 days (92.3%), in 4 patients foot ulcers were better after 4-12 weeks. ABI increased in 6 cases. The total effective rate was 92.3%. No complication or adverse effect was observed in all the patients. Conclusion Autologous PBSC transplantation might be a safe and effective method for ASO of the lower limb ASO.