Mechanism of mitochondrial dysfunction and ultrastructural changes induced by rotenone in PC12 cells / 第三军医大学学报

ABSTRACT

Objective To observe the effects of different doses of rotenone on reactive oxygen species, mitochondrial transemembrane potential as well as mitochondrial ultrastructure in order to investigate the possible mechanism of mitochondrial dysfunction in cultured pheochromaffinoma cells. Methods ROS production induced by rotenone in cells were measured using flow cytometry. Mitochondrial transmembrane potential was detected by laser scanning confocal microscope and mitochondrial ultrastructures were observed under transmission electron microscope. Results Treatment with 0.1, 1.0, 2.0 and 3.0 ?mol/L concentrations of rotenone could increase the generation of ROS in PC12 cells as 1.55?0.17, 2.16?0.10, 1.77?0.20 and 1.40?0.12, and disrupt the mitochondrial transmembrane potential about 93.86?10.12, 119.43?7.09, 102.71?9.36 and 83.14?10.70. There were significant differences as compared with the control group (P