Effects of Qilantangzhining Capsule on Hepatocyte Apoptosis and Expression of Bax and Bcl-2 in Rats with Diabetes Mellitus and Hyperlipemia / 中华中医药杂志

ABSTRACT

Objective: To study the mechanism of Qilantangzhining Capsule in treating diabetes mellitus complicated with hyperlipemia. Method: Rats were fed with high sugar and fat food for 2 months, and then 2% streptozocin (STZ, 30mg/kg) was injected intraperitoneally to induce hyperglycemic and hyperlipemic model. Thereafter, the model rats were administrated the drug. Flow cytometry (FCM) were used to detect effects of the capsule on hepatocyte apoptosis and the expression of related regulating genes, Bax and Bcl-2. Result: The rate of cell apoptosis remarkably increased among the model rats, the expression of Bax was significantly enhanced and that of Bcl-2 was decreased. Qilantangzhining Capsule could remarkably decrease the rate of cell apoptosis, decrease the expression of Bax, and increase that of Bcl-2. Conclusion: Qilantangzhining Capsule can prevent hepatocyte apoptosis by adjust the expression of Bax and Bcl-2, and accordingly provide evidences for its role in adjusting the metabolic disorders of sugar and fat.