THE PROTECTIVE EFFECT OF POLYSACCHARIDES FROM ROSE ROXBURGHII TRATT ON NEURAL STEM CELLS DEMAGED BY GLUTAMIC ACID / 营养学报

ABSTRACT

Objective:To study the protective effect of polysaccharide from Rose roxburghii Tratt (RRTP-1) on neural stem cells damaged by glutamic acid (Glu). Method: RRTP-1 was obtained by ion exchange column chromatography and gel chromatography. The neural stem cells were cultured from the striata of fetal mice and damaged by glutamic acid to simulate hypoxic-ischemic brain injury. The cultures were divided into control group, glutamic acid group and test groups in which different doses of RRTP-1 were added with glutamic acid. The death rate of neural stem cells damaged by Glu and LDH leakage were observed. Results:The neural stem cells damage of test groups were alleviated,and the death rate of neural stem cells and LDH leakage of high dose test group were significantly lower than glutamic acid group. Conclusion:RRTP-1 has remarkable protective effect on neural stem cells damage.