EFFECT OF BLACK CURRANT ON PROLIFERATION AND APOPTOSIS OF HUMAN ESOPHAGEAL CANCER CELL LINE IN VITRO / 营养学报
Acta Nutrimenta Sinica
;
(6)2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-557388
ABSTRACT
Objective:
To investigate the effect of black currant extract on human esophageal squamous cancer cell Eca109 line to reveal its probable mechanism.Methods:
The survival cells and protein synthesis of tumor cell lines treated with 10-1, 10 -2,10-3 g/ml of the water extracts of black currant for 24 h were determined by MTT and Bradford assays, and the cell-DNA ploidy distribution and apoptotic rate were measured by flow cytometry (FCM) and morphological observation.Results:
There was significant inhibition of the cell survival and protein synthesis of cancer cells at dose of 10-1 g/ml of the extracts. In the normal rat liver cells, the cell survival was not affected. An apoptosis peak appeared before diploid peak in FCM and apoptotic rate was 49.6%, and morphological change of apoptosis was observed.Conclusion:
Black currant could significantly inhibit growth of human esophageal squamous cancer cell Eca109 line through inducing apoptosis.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Acta Nutrimenta Sinica
Ano de publicação:
2004
Tipo de documento:
Artigo
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