Immune response to pT-ureB and pT-hpaA DNA vaccine in mice / 第三军医大学学报

ABSTRACT

Objective To investigate and compare the humoral immune response induced by eukaryotic expression plasmid pTCAEureB (pTureB)and pTCAEhpaA (pThpaA) injected intramuscularly in mice. Methods The ureB gene fragment was inserted into a eukaryotic expression vector pTCAE, and pTCAEureB was constructed. A total of 60 BALB/c mice were randomly assigned to be immunized by intramuscular injection of blank plasmid pTCAE, plasmid pTureB and pThpaA (n=20 in each group). Titers of specific IgG antibody in serum of each group were detected through ELISA respectively on week 2, 4, 6 after immunization. Results Specific IgG were observed in pTureB and pThpaA immunized groups and titers of antibody increased as time prolonged. IgG level was significantly higher in pT-ureB immunized group than in pT-hpaA group on week 6 after immunization. Conclusion Plasmid pT-ureB possesses good immunogenicity and induces a higher immune response than pT-hpaA.